Sunday, August 20, 2017

Review Questions on Instrumentation and Venipuncture for Clinical Chemistry

1. Particulate formation is a problem in analysis of:
a. ultraviolet  spectrophotometry            d. flame photometry
b. fluorescence                    e. NIL
c. atomic absorption

2. For photometric measurements below 320 mu, the cuvette must be:
a. quartz                        d. NaCl
b. tungsten glass                    e. Pyrex glass
c. borosilicate glass

3. Nephelometry is used to measure:
a.    ultraviolet absorbing material
b.    infrared absorbing material
c.    particles
d.    colored solutions
e.    NIL

4. The gram-molecular weight per liter of solution is:
a.  Molarity                    c.  Normality        e. NIL
b.  Molality                    d.  Percent

5.  It involves the preparation of a weaker solution from a stronger solution:
a.  serial dilution                c.  dilution        e. NIL
b.  percent                    d.  gravimetry

6. To solve for the dilution of a solution, you have to:
a.  multiply all the number of parts of the diluent with the original concentration
b.  add the number of parts of the solute and the solvent
c.  add all the numerators and denominators
d.  add all the volumes and multiply by the original concentration
e. NIL

7. Which of the following formula correctly describes the relationship between absorbance  and % T ?
a. A= 2- log % T                    c. A= - log T
b. A = log 1/T                        d. all of the above
                            e. NIL

8. A chopper is used in a dual beam spectrophotometer in order to:
a.    reduce the number of moving  parts
b.    facilitate wavelength  scanning
c.    obviate the need for matched detectors
d.    reduce stray light
e.    NIL

9. The reagent blank corrects for absorbance caused by:
a.    the color of reagents
b.    sample turbidity
c.    bilirubin and hemolysis
d.    all of the above
e.    NIL

10. Which instrument  requires a primary and secondary monochromator?
a.    flame photometer
b.    atomic absorption
c.    fluorometer
d.    nephelometer
e.    NIL

11. All of the following statements about fluorometry are true, except:
a.    fluorometry is more sensitive than spectrophotometry
b.    both excitation and emission spectra are characteristics  of the analyte
c.    fluorescence  is directly proportional to temperature
d.    unsaturated  cyclic molecules are often fluorescent
e.    NIL

12. The ideal anticoagulant because of its natural presence in the blood:
a. citrate                        c. oxalate        e. NIL
b. heparin                        d. fluoride

13. The anticoagulant of choice for glucose because it serves as preservative :
Select from the above choices

14. The most common site of venipuncture:
a. wrist veins                        c. ankle veins        e. NIL
b. antecubital fossa                    d. cubital  veins

15. The following are sites of arterial puncture, except:
a. radial                        c. brachial        e. NIL
b. antecubital  fossa                    d. femoral

16. The ideal gauge of needle for venipuncture is:
a. 22- 25                        c. 21-23        e. NIL
b. 20-22                        d. 18-20

17. It is the law applied to spectrophotometry:
a.  Beer-Lambert’s Law            c.  Kirchoff’s Law
b.  Newton’s Law                d.  Absorptivity Coeffecient
                                e. NIL

18. The following are basic components of the  spectrophotometer, except;
        a.  burner assembly                c.  cuvette               e. NIL
        b.  monochomator                d.  exit slit

19.    The more light absorbed, the higher  the concentration of analyte in this technique of measuring the amount of light absorbed  by a solution:
a.    atomic absorption                d. fluorometry      
b.    nephelometry                e. NIL
c.    spectrophotometry                    

20. The basis of EFP  is the measurement of:
a.    light given off by excited atoms
b.    light absorbed at wavelength of resonance line by dissociated atoms
c.    energy emitted by ultraviolet treated atoms
d.    energy emitted by infrared treated atoms
e.    NIL

21. 2- log T is a definition of (T= transmission):
    a. absorbance                d. electron density        e. NIL
     b. ionic strength                e. electrophoretic density

22.In Osmometry, concentration of substance can be measured by:
   a. colorimetry                d. freezing point depression
    b. appropriate filters            e. NIL
    c. ionized atoms

23.Quenching is a problem in analysis of:
   a. ultraviolet  spectrophotometry        d. flame photometry
   b. fluorescence                e. NIL
   c. atomic absorption

24. For AAS measurements the light source is usually the:
a. hollow cathode lamp                d. Tungsten light bulb
b. Deuterium lamp                    e. NIL
c. halogen lamp

25.Electrophoresis is used to measure :
a.    ultraviolet substances                e. NIL
b.    infrared absorbing materials
c.    charged particles
d.    colored solutions

26.The water blank corrects for absorbance caused by:
a.    the color of reagents                 e. NIL
b.    sample turbidity
c.    bilirubin and hemolysis
d.    all of the above

27. The internal standards in EFP, are:
a.    Didymium and lithium
b.    Cesium and lithium
c.    Potassium and didymium
d.    Cesium and didymium
e.    NIL

28.    The parts of the burner assembly are the following:
a.    Aspirator, atomizer, flame
b.    Aspirator, ionizer, flame
c.    Atomizer, emitter, flame
d.    Atomizer, ionizer, flame
e.    NIL

29.    The most accurate vessel in volumetric measurements is the:
a.    Erlenmeyer flask
b.    Graduated cylinder
c.    Serological pipette
d.    Volumetric flask
e.    NIL

30.    Volumetric measurements are generally used in:
a.    Liquid preparations only
b.    Solid and liquid preparations
c.    Solid preparations only
d.    AIL
e.    NIL

31.    The unit of volume in volumetric measurements is:
a.    Deciliter
b.    Micriolter
c.    Milliliter
d.    Liter
e.    NIL

32.    In electrophoresis, the basis of separation are the following, EXCEPT:
a.    Charges of ions
b.    Electrophoretic media
c.    Net charge of particles
d.    Concentration of ions
e.    NIL

33.    The movement of ions in electrophoresis is from:
a.    Negative to positive
b.    Positive to negative
c.    Negative to negative
d.    Positive to positive
e.    NIL

34.    The principle of chromatography is:
a.    The movement of charged particles in an electric field
b.    The involvement the solute and solvent
c.    The measurement of emitted light
d.    The excitation of ions in solution
e.    NIL

35.    Chromatography is affected by the following factors, EXCEPT:
a.    Density of particles in solution
b.    Size of particle
c.    Affinity of particles to chromatographic media
d.    pH
e.    NIL

36.    The following precautions are observed in Gravimetry, EXCEPT:
a.    The balance should be adjusted to zero
b.    The substance to be measured should be hydrated
c.    The substance should be uncontaminated
d.    The vessels used should be clean and dry
e.    NIL


No comments:





Except for the outside links, printing and copying of the articles contained herein should cite this site as the source. For commercial purposes, the permission of the author should be sought first. You can leave a comment in any of the posts. Thank you.